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normal murine fibroblast cell line nih 3t3  (ATCC)


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    Structured Review

    ATCC normal murine fibroblast cell line nih 3t3
    Cytotoxic and anti-cancer activity of the N1: (A) dose-dependent inhibition of cell proliferation in murine colon carcinoma cell lines (CT26 and MC-38) and human colorectal cancer cell line (HCT-15) upon treatment with increasing concentrations of N1, determined by MTT assay; (B) cell viability of normal murine fibroblast cells <t>(NIH-3T3)</t> following treatment with N1 at indicated concentrations, demonstrating minimal cytotoxicity; (C–E) dose–response curves showing percentage growth inhibition and corresponding IC 50 values for CT26 (C), MC-38 (D), and HCT-15 (E) cells. IC 50 values are calculated by nonlinear regression analysis using GraphPad Prism. Data are expressed as mean ± SD ( n = 3).
    Normal Murine Fibroblast Cell Line Nih 3t3, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 13151 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/normal+murine+fibroblast+cell+line+nih+3t3/NIH%2F3T3/pmc13045661-49-14-23
    Average 99 stars, based on 13151 article reviews
    normal murine fibroblast cell line nih 3t3 - by Bioz Stars, 2026-09
    99/100 stars

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    1) Product Images from "Exploring an azo-uracil based nickel( ii ) complex for anticancer and phosphatase like activities"

    Article Title: Exploring an azo-uracil based nickel( ii ) complex for anticancer and phosphatase like activities

    Journal: RSC Advances

    doi: 10.1039/d6ra01587e

    Cytotoxic and anti-cancer activity of the N1: (A) dose-dependent inhibition of cell proliferation in murine colon carcinoma cell lines (CT26 and MC-38) and human colorectal cancer cell line (HCT-15) upon treatment with increasing concentrations of N1, determined by MTT assay; (B) cell viability of normal murine fibroblast cells (NIH-3T3) following treatment with N1 at indicated concentrations, demonstrating minimal cytotoxicity; (C–E) dose–response curves showing percentage growth inhibition and corresponding IC 50 values for CT26 (C), MC-38 (D), and HCT-15 (E) cells. IC 50 values are calculated by nonlinear regression analysis using GraphPad Prism. Data are expressed as mean ± SD ( n = 3).
    Figure Legend Snippet: Cytotoxic and anti-cancer activity of the N1: (A) dose-dependent inhibition of cell proliferation in murine colon carcinoma cell lines (CT26 and MC-38) and human colorectal cancer cell line (HCT-15) upon treatment with increasing concentrations of N1, determined by MTT assay; (B) cell viability of normal murine fibroblast cells (NIH-3T3) following treatment with N1 at indicated concentrations, demonstrating minimal cytotoxicity; (C–E) dose–response curves showing percentage growth inhibition and corresponding IC 50 values for CT26 (C), MC-38 (D), and HCT-15 (E) cells. IC 50 values are calculated by nonlinear regression analysis using GraphPad Prism. Data are expressed as mean ± SD ( n = 3).

    Techniques Used: Activity Assay, Inhibition, MTT Assay

    Related Articles

    Activity Assay:

    Article Title: Exploring an azo-uracil based nickel( ii ) complex for anticancer and phosphatase like activities
    Article Snippet: Cell culture reagents, including Dulbecco's Modified Eagle Medium (DMEM), fetal bovine serum (FBS), penicillin–streptomycin, and trypsin-EDTA, were obtained from Gibco (Thermo Fisher Scientific, USA).Cell culture reagents, including Dulbecco's Modified Eagle Medium (DMEM), fetal bovine serum (FBS), penicillin–streptomycin, and trypsin-EDTA, were obtained from Gibco (Thermo Fisher Scientific, USA).. The murine colon carcinoma cell lines CT26, human colorectal cancer cell line HCT-15, and normal murine fibroblast cell line NIH-3T3 were procured from ATCC (USA).. For biological assays, Annexin V-FITC/propidium iodide (PI) apoptosis detection kit, CellROXTM Green reagent, MitoSOXTM Red mitochondrial superoxide indicator, JC-1 mitochondrial membrane potential probe, and LDH cytotoxicity assay kit were obtained from Invitrogen (USA) and used according to the manufacturer's instructions.For biological assays, Annexin V-FITC/propidium iodide (PI) apoptosis detection kit, CellROXTM Green reagent, MitoSOXTM Red mitochondrial superoxide indicator, JC-1 mitochondrial membrane potential probe, and LDH cytotoxicity assay kit were obtained from Invitrogen (USA) and used according to the manufacturer's instructions.

    Article Title: Exploring an azo-uracil based nickel( ii ) complex for anticancer and phosphatase like activities
    Article Snippet: Murine colon carcinoma cell lines CT26 and MC-38, human colorectal carcinoma cell line HCT-15, and normal murine fibroblast cell line NIH-3T3 were procured from the ATCC, USA.. All cell lines were cultured in Dulbecco's Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 1% penicillin–streptomycin, and maintained in a humidified incubator at 37 °C with 5% CO 2 atmosphere.All cell lines were cultured in Dulbecco's Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 1% penicillin–streptomycin, and maintained in a humidified incubator at 37 °C with 5% CO 2 atmosphere.

    Inhibition:

    Article Title: Exploring an azo-uracil based nickel( ii ) complex for anticancer and phosphatase like activities
    Article Snippet: Cell culture reagents, including Dulbecco's Modified Eagle Medium (DMEM), fetal bovine serum (FBS), penicillin–streptomycin, and trypsin-EDTA, were obtained from Gibco (Thermo Fisher Scientific, USA).Cell culture reagents, including Dulbecco's Modified Eagle Medium (DMEM), fetal bovine serum (FBS), penicillin–streptomycin, and trypsin-EDTA, were obtained from Gibco (Thermo Fisher Scientific, USA).. The murine colon carcinoma cell lines CT26, human colorectal cancer cell line HCT-15, and normal murine fibroblast cell line NIH-3T3 were procured from ATCC (USA).. For biological assays, Annexin V-FITC/propidium iodide (PI) apoptosis detection kit, CellROXTM Green reagent, MitoSOXTM Red mitochondrial superoxide indicator, JC-1 mitochondrial membrane potential probe, and LDH cytotoxicity assay kit were obtained from Invitrogen (USA) and used according to the manufacturer's instructions.For biological assays, Annexin V-FITC/propidium iodide (PI) apoptosis detection kit, CellROXTM Green reagent, MitoSOXTM Red mitochondrial superoxide indicator, JC-1 mitochondrial membrane potential probe, and LDH cytotoxicity assay kit were obtained from Invitrogen (USA) and used according to the manufacturer's instructions.

    Article Title: Exploring an azo-uracil based nickel( ii ) complex for anticancer and phosphatase like activities
    Article Snippet: Murine colon carcinoma cell lines CT26 and MC-38, human colorectal carcinoma cell line HCT-15, and normal murine fibroblast cell line NIH-3T3 were procured from the ATCC, USA.. All cell lines were cultured in Dulbecco's Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 1% penicillin–streptomycin, and maintained in a humidified incubator at 37 °C with 5% CO 2 atmosphere.All cell lines were cultured in Dulbecco's Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 1% penicillin–streptomycin, and maintained in a humidified incubator at 37 °C with 5% CO 2 atmosphere.

    MTT Assay:

    Article Title: Exploring an azo-uracil based nickel( ii ) complex for anticancer and phosphatase like activities
    Article Snippet: Cell culture reagents, including Dulbecco's Modified Eagle Medium (DMEM), fetal bovine serum (FBS), penicillin–streptomycin, and trypsin-EDTA, were obtained from Gibco (Thermo Fisher Scientific, USA).Cell culture reagents, including Dulbecco's Modified Eagle Medium (DMEM), fetal bovine serum (FBS), penicillin–streptomycin, and trypsin-EDTA, were obtained from Gibco (Thermo Fisher Scientific, USA).. The murine colon carcinoma cell lines CT26, human colorectal cancer cell line HCT-15, and normal murine fibroblast cell line NIH-3T3 were procured from ATCC (USA).. For biological assays, Annexin V-FITC/propidium iodide (PI) apoptosis detection kit, CellROXTM Green reagent, MitoSOXTM Red mitochondrial superoxide indicator, JC-1 mitochondrial membrane potential probe, and LDH cytotoxicity assay kit were obtained from Invitrogen (USA) and used according to the manufacturer's instructions.For biological assays, Annexin V-FITC/propidium iodide (PI) apoptosis detection kit, CellROXTM Green reagent, MitoSOXTM Red mitochondrial superoxide indicator, JC-1 mitochondrial membrane potential probe, and LDH cytotoxicity assay kit were obtained from Invitrogen (USA) and used according to the manufacturer's instructions.

    Article Title: Exploring an azo-uracil based nickel( ii ) complex for anticancer and phosphatase like activities
    Article Snippet: Murine colon carcinoma cell lines CT26 and MC-38, human colorectal carcinoma cell line HCT-15, and normal murine fibroblast cell line NIH-3T3 were procured from the ATCC, USA.. All cell lines were cultured in Dulbecco's Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 1% penicillin–streptomycin, and maintained in a humidified incubator at 37 °C with 5% CO 2 atmosphere.All cell lines were cultured in Dulbecco's Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 1% penicillin–streptomycin, and maintained in a humidified incubator at 37 °C with 5% CO 2 atmosphere.



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    Cytotoxic and anti-cancer activity of the N1: (A) dose-dependent inhibition of cell proliferation in murine colon carcinoma cell lines (CT26 and MC-38) and human colorectal cancer cell line (HCT-15) upon treatment with increasing concentrations of N1, determined by MTT assay; (B) cell viability of normal murine fibroblast cells <t>(NIH-3T3)</t> following treatment with N1 at indicated concentrations, demonstrating minimal cytotoxicity; (C–E) dose–response curves showing percentage growth inhibition and corresponding IC 50 values for CT26 (C), MC-38 (D), and HCT-15 (E) cells. IC 50 values are calculated by nonlinear regression analysis using GraphPad Prism. Data are expressed as mean ± SD ( n = 3).
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    Image Search Results


    Cytotoxic and anti-cancer activity of the N1: (A) dose-dependent inhibition of cell proliferation in murine colon carcinoma cell lines (CT26 and MC-38) and human colorectal cancer cell line (HCT-15) upon treatment with increasing concentrations of N1, determined by MTT assay; (B) cell viability of normal murine fibroblast cells (NIH-3T3) following treatment with N1 at indicated concentrations, demonstrating minimal cytotoxicity; (C–E) dose–response curves showing percentage growth inhibition and corresponding IC 50 values for CT26 (C), MC-38 (D), and HCT-15 (E) cells. IC 50 values are calculated by nonlinear regression analysis using GraphPad Prism. Data are expressed as mean ± SD ( n = 3).

    Journal: RSC Advances

    Article Title: Exploring an azo-uracil based nickel( ii ) complex for anticancer and phosphatase like activities

    doi: 10.1039/d6ra01587e

    Figure Lengend Snippet: Cytotoxic and anti-cancer activity of the N1: (A) dose-dependent inhibition of cell proliferation in murine colon carcinoma cell lines (CT26 and MC-38) and human colorectal cancer cell line (HCT-15) upon treatment with increasing concentrations of N1, determined by MTT assay; (B) cell viability of normal murine fibroblast cells (NIH-3T3) following treatment with N1 at indicated concentrations, demonstrating minimal cytotoxicity; (C–E) dose–response curves showing percentage growth inhibition and corresponding IC 50 values for CT26 (C), MC-38 (D), and HCT-15 (E) cells. IC 50 values are calculated by nonlinear regression analysis using GraphPad Prism. Data are expressed as mean ± SD ( n = 3).

    Article Snippet: The murine colon carcinoma cell lines CT26, human colorectal cancer cell line HCT-15, and normal murine fibroblast cell line NIH-3T3 were procured from ATCC (USA).

    Techniques: Activity Assay, Inhibition, MTT Assay